The FISH ( situ hybridization) in spermatozoa is an analysis of possible alterations in some of their chromosomes. This test can be indicated for abnormal seminograms, if the male's karyotype is altered, in recurrent miscarriages, or in implantation failures .
During the formation of spermatozoa in the testicles, errors may occur that an extra copy of some chromosome (disomy), without any chromosome (monosomy) will give rise to spermatozoa >or with all duplicated chromosomes (diploidy). If any of these abnormal spermatozoa were to fertilize the oocyte, it could lead to non-implantation of the resulting embryo or abortions. The number of chromosomes to be studied (3, 4, 5 or 9 chromosomes), the number of spermatozoa counted (from a few hundred to thousands) and whether they are carried out automatically (computer) or manually (by an operator), determine the reliability and quality of the result achieved.
At URH García del Real, thanks to our collaboration with an external genetics laboratory with extensive experience in this technique (Sistemas Genómicos ), FISH is performed on sperm that analyzes the largest possible number of chromosomes (9) through a automated system that observes 10,000 sperm. In this way we obtain reliable and reproducible results and in the shortest possible time.
This test is performed on a semen sample obtained by masturbation. It is recommended to obtain the sample in the clinic, where you will be provided with the appropriate place and the sterile bottle for it. You must be sexually abstinent for 3 to 7 days before testing. It is necessary to make an appointment directly with the IVF Laboratory staff. This test can be taken from Monday to Friday from 8:00 a.m. to 4:00 p.m.. The results will be available in 3 -4 weeks.